Corticosteroid Biosynthesis in the Interrenal Cells of the Teleost Fish, Oreochromis mossambicus

Corticosteroid biosynthesis in the interrenal cells of the teleost fish, Oreochromis mossambicus.

Applying high-performance liquid chromatography and thin-layer chromatography to separate corticosteroids, we studied the biosynthesis of steroids by the interrenal cells of the head kidneys (the adrenocortical homolog) of Oreochromis mossambicus. Intact head kidneys converted exogenous 17 alpha-hydroxyprogesterone into mainly cortisol, but 11-deoxycortisol, cortisone, and androstenedione were ...

Effects of calcium and phosphate on the corpuscles of Stannius of the teleost fish, Oreochromis mossambicus

The impacts of neutralized acid mine drainage contaminated water on the expression of selected endocrine-linked genes in juvenile Mozambique tilapia Oreochromis mossambicus exposed in vivo.

Acid mine drainage (AMD) is a global environmental concern due to detrimental impacts on river ecosystems. Little is however known regarding the biological impacts of neutralized AMD on aquatic vertebrates despite excessive discharge into watercourses. The aim of this investigation was to evaluate the endocrine modulatory potential of neutralized AMD, using molecular biomarkers in the teleost f...

Superfusion of pituitary tissue

Tilapia (Oreochromis mossambicus] teleostei) melanin-concentrating hormone gene-related peptide (tMgrp) was tested for tropic actions on adenocorticotropin hormone (ACTH) and a melanocyte s t im u la t in g ho rm one ( a M S H ) producing cells in the tilapia pituitary gland in vitro. Up to 100 pM synthetic tilapia Mgrp (tMgrp) had no effect on a -M S H release from tilapia neurointermediate lo...

Corticotropin-releasing hormone in the teleost stress response: rapid appearance of the peptide in plasma of tilapia (Oreochromis mossambicus).

High concentrations (up to 600 pg/ml) of corticotropin-releasing hormone (CRH) were detected in plasma of the teleost fish Oreochromis mossambicus (tilapia) when screening peripheral tissues of tilapia exposed to stress. Notably, the plasma CRH response to stressors in tilapia is much more pronounced than that in higher vertebrates, such as rats. After characterisation by RIA, by spiking plasma...